The NFL, Cardinals, and Minnesota Vikings, all released comments on Monday announcing the loss.
Gladney was killed in an early-morning accident in the Dallas area.
According to the Fort Worth Star-Telegram, Houston-based Brian Overstreet also cited a crash.
Overstreet did not respond immediately to requests for additional information.
Gladney was one of two people killed in a crash involving a Mercedes-Benz SUV, according to NBC Dallas-Fort Worth.
Overnight on a Dallas service road for the Woodall Rodgers Freeway.
Mercedes Palacios, 26, was also declared dead at the scene, according to her family. According to the report, she was a passenger in the SUV.
According to NBC Dallas-Fort Worth, speed may have played a role, citing officials whose investigation was ongoing.
In 2020, the Vikings selected him in the first round. He agreed to terms with the Cardinals earlier this year.
Gladney was from New Boston, Texas, and the university described him as "one of the best high school cornerbacks in the state."